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| Acceso al texto completo restringido a Biblioteca INIA La Estanzuela. Por información adicional contacte bib_le@inia.org.uy. |
Registro completo
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Biblioteca (s) : |
INIA La Estanzuela. |
Fecha : |
18/12/2020 |
Actualizado : |
18/12/2020 |
Tipo de producción científica : |
Artículos en Revistas Indexadas Internacionales |
Autor : |
COPPOLA, N.; FREIRE, B.; UMPIÉRREZ, A.; CORDEIRO, N.F.; AVILA. P.; TRENCHI, G.; CASTRO, G.; CASAUX, M.L.; FRAGA, M.; ZUNINO, P.; BADO, I.; VIGNOLI, R. |
Afiliación : |
NADIA COPPOLA, Departamento de Bacteriología y Virología, Facultad de Medicina, Universidad de la República, Montevideo, Uruguay.; BIBIANA FREIRE, Unidad Académica Animales de Granja, Facultad de Veterinaria, Universidad de la República, Montevideo, Uruguay.; ANA UMPIÉRREZ, Departamento de Microbiología, Instituto de Investigaciones Biológicas Clemente Estable, Montevideo, Uruguay.; NICOLÁS F. CORDEIRO, Departamento de Bacteriología y Virología, Facultad de Medicina, Universidad de la República, Montevideo, Uruguay.; PABLO AVILA, Departamento de Bacteriología y Virología, Facultad de Medicina, Universidad de la República, Montevideo, Uruguay.; GUSTAVO TRENCHI, Veterinario de Libre Ejercicio, Montevideo, Uruguay.; GUSTAVO CASTRO, Unidad Académica Animales de Granja, Facultad de Veterinaria, Universidad de la República, Montevideo, Uruguay.; MARÍA LAURA CASAUX, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay; MARTIN FRAGA COTELO, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay; PABLO ZUNINO, Departamento de Microbiología, Instituto de Investigaciones Biológicas Clemente Estable, Montevideo, Uruguay.; INÉS BADO, Departamento de Bacteriología y Virología, Facultad de Medicina, Universidad de la República, Montevideo, Uruguay; RAFAEL VIGNOLI, Departamento de Bacteriología y Virología, Facultad de Medicina, Universidad de la República, Montevideo, Uruguay. |
Título : |
Transferable Resistance to Highest Priority Critically Important Antibiotics for Human Health in Escherichia coli Strains Obtained From Livestock Feces in Uruguay. (Brief Research Report Article). |
Fecha de publicación : |
2020 |
Fuente / Imprenta : |
Frontiers in Veterinary Science, 19 November 2020, Volume 7, Article number 588919. OPEN ACCESS. Doi: https://doi.org/10.3389/fvets.2020.588919 |
DOI : |
10.3389/fvets.2020.588919 |
Idioma : |
Inglés |
Notas : |
Article history: Received: 29 July 2020/ Accepted: 19 October 2020/Published: 19 November 2020. Correspondence: Rafael Vignoli, rvignoli@higiene.edu.uy; Inés Bado, ibado@higiene.edu.uy |
Contenido : |
Abstract: The aim of this work was to detect Escherichia coli isolates displaying resistance to oxyimino-cephalosporins, quinolones, and colistin in feces from livestock in Uruguay. During 2016?2019, fecal samples from 132 broiler and layer chicken flocks, 100 calves, and 50 pigs, were studied in Uruguay. Samples were cultured on MacConkey Agar plates supplemented with ciprofloxacin, ceftriaxone, or colistin. E. coli isolates were identified by mass spectrometry and antibiotic susceptibility testing was performed by disk diffusion agar method and colistin agar test. Antibiotic resistance genes were detected by polymerase chain reaction and sequencing. The most frequently detected resistance gene was qnrB19, recovered from 87 animals. Regarding plasmid-mediated quinolone resistance genes, qnrS1 was the second in prevalence (23 animals) followed by qnrE1, found in 6 chickens and two calves. Regarding resistance to oxyimino-cephalosporins, 8 different ?-lactamase genes were detected: blaCTX?M?8 and blaCMY?2 were found in 23 and 19 animals, respectively; next, blaCTX?M?2 and blaSHV?12 in 7 animals each, followed by blaCTX?M?14 in 5, blaCTX?M?15 and blaSHV2a in 2, and blaCTX?M?55 in a single animal. Finally, the mcr-1 gene was detected only in 8 pigs from a single farm, and in a chicken. Isolates carrying blaCMY?2 and blaSHV?12 were also found in these animals, including two isolates featuring the blaCMY?2/mcr-1 genotype. To the best of our knowledge, this is the first work in which the search for transferable resistance to highest priority critically important antibiotics for human health is carried out in chickens and pigs chains of production animals in Uruguay. MenosAbstract: The aim of this work was to detect Escherichia coli isolates displaying resistance to oxyimino-cephalosporins, quinolones, and colistin in feces from livestock in Uruguay. During 2016?2019, fecal samples from 132 broiler and layer chicken flocks, 100 calves, and 50 pigs, were studied in Uruguay. Samples were cultured on MacConkey Agar plates supplemented with ciprofloxacin, ceftriaxone, or colistin. E. coli isolates were identified by mass spectrometry and antibiotic susceptibility testing was performed by disk diffusion agar method and colistin agar test. Antibiotic resistance genes were detected by polymerase chain reaction and sequencing. The most frequently detected resistance gene was qnrB19, recovered from 87 animals. Regarding plasmid-mediated quinolone resistance genes, qnrS1 was the second in prevalence (23 animals) followed by qnrE1, found in 6 chickens and two calves. Regarding resistance to oxyimino-cephalosporins, 8 different ?-lactamase genes were detected: blaCTX?M?8 and blaCMY?2 were found in 23 and 19 animals, respectively; next, blaCTX?M?2 and blaSHV?12 in 7 animals each, followed by blaCTX?M?14 in 5, blaCTX?M?15 and blaSHV2a in 2, and blaCTX?M?55 in a single animal. Finally, the mcr-1 gene was detected only in 8 pigs from a single farm, and in a chicken. Isolates carrying blaCMY?2 and blaSHV?12 were also found in these animals, including two isolates featuring the blaCMY?2/mcr-1 genotype. To the best of our knowledge, this is the first work in wh... Presentar Todo |
Palabras claves : |
CMY-2; CTX-M-8; E. COLI; ESBL; ESCHERICHIA COLI; MCR-1; PLATAFORMA SALUD ANIMAL; POULTRY; QNRB19; SWINE. |
Thesagro : |
AVES DE CORRAL; CERDO. |
Asunto categoría : |
-- |
Marc : |
LEADER 03119nam a2200409 a 4500 001 1061590 005 2020-12-18 008 2020 bl uuuu u0uu1 u #d 024 7 $a10.3389/fvets.2020.588919$2DOI 100 1 $aCOPPOLA, N. 245 $aTransferable Resistance to Highest Priority Critically Important Antibiotics for Human Health in Escherichia coli Strains Obtained From Livestock Feces in Uruguay. (Brief Research Report Article).$h[electronic resource] 260 $aFrontiers in Veterinary Science, 19 November 2020, Volume 7, Article number 588919. OPEN ACCESS. Doi: https://doi.org/10.3389/fvets.2020.588919$c2020 500 $aArticle history: Received: 29 July 2020/ Accepted: 19 October 2020/Published: 19 November 2020. Correspondence: Rafael Vignoli, rvignoli@higiene.edu.uy; Inés Bado, ibado@higiene.edu.uy 520 $aAbstract: The aim of this work was to detect Escherichia coli isolates displaying resistance to oxyimino-cephalosporins, quinolones, and colistin in feces from livestock in Uruguay. During 2016?2019, fecal samples from 132 broiler and layer chicken flocks, 100 calves, and 50 pigs, were studied in Uruguay. Samples were cultured on MacConkey Agar plates supplemented with ciprofloxacin, ceftriaxone, or colistin. E. coli isolates were identified by mass spectrometry and antibiotic susceptibility testing was performed by disk diffusion agar method and colistin agar test. Antibiotic resistance genes were detected by polymerase chain reaction and sequencing. The most frequently detected resistance gene was qnrB19, recovered from 87 animals. Regarding plasmid-mediated quinolone resistance genes, qnrS1 was the second in prevalence (23 animals) followed by qnrE1, found in 6 chickens and two calves. Regarding resistance to oxyimino-cephalosporins, 8 different ?-lactamase genes were detected: blaCTX?M?8 and blaCMY?2 were found in 23 and 19 animals, respectively; next, blaCTX?M?2 and blaSHV?12 in 7 animals each, followed by blaCTX?M?14 in 5, blaCTX?M?15 and blaSHV2a in 2, and blaCTX?M?55 in a single animal. Finally, the mcr-1 gene was detected only in 8 pigs from a single farm, and in a chicken. Isolates carrying blaCMY?2 and blaSHV?12 were also found in these animals, including two isolates featuring the blaCMY?2/mcr-1 genotype. To the best of our knowledge, this is the first work in which the search for transferable resistance to highest priority critically important antibiotics for human health is carried out in chickens and pigs chains of production animals in Uruguay. 650 $aAVES DE CORRAL 650 $aCERDO 653 $aCMY-2 653 $aCTX-M-8 653 $aE. COLI 653 $aESBL 653 $aESCHERICHIA COLI 653 $aMCR-1 653 $aPLATAFORMA SALUD ANIMAL 653 $aPOULTRY 653 $aQNRB19 653 $aSWINE 700 1 $aFREIRE, B. 700 1 $aUMPIÉRREZ, A. 700 1 $aCORDEIRO, N.F. 700 1 $aAVILA. P. 700 1 $aTRENCHI, G. 700 1 $aCASTRO, G. 700 1 $aCASAUX, M.L. 700 1 $aFRAGA, M. 700 1 $aZUNINO, P. 700 1 $aBADO, I. 700 1 $aVIGNOLI, R.
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| Acceso al texto completo restringido a Biblioteca INIA Las Brujas. Por información adicional contacte bibliolb@inia.org.uy. |
Registro completo
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Biblioteca (s) : |
INIA Las Brujas. |
Fecha actual : |
21/02/2014 |
Actualizado : |
24/01/2019 |
Tipo de producción científica : |
Artículos en Revistas Indexadas Internacionales |
Circulación / Nivel : |
Internacional - -- |
Autor : |
CARBALLO, S.; BLANKENSHIP, S. M.; SANDERS, D. C.; RITCHIE, D. F. |
Afiliación : |
SERGIO JOSE CARBALLO SILVA, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay; SYLVIA M. BLANKENSHIP; DOUGLAS C. SANDERS; DAVID F. RITCHIE. |
Título : |
Drip fertigation with nitrogen and potassium and postharvest susceptibility to bacterial soft rot of bell peppers. |
Fecha de publicación : |
1994 |
Fuente / Imprenta : |
Journal of Plant Nutrition, 1994, volume 17, Issue 7, pages 1175-1191. |
DOI : |
10.1080/01904169409364797 |
Idioma : |
Inglés |
Notas : |
Article history: Published online: 21 Nov 2008.
Partial funding for this research was from Instituto Nacional de Investigacion Agropecuaria of Uruguay (I.N.I.A.), Uruguay and from the Unites States Department of Agriculture Special Grant F.L. 89-106: "Agricultural Adjustment in the Southeast Through Alternative Cropping Systems." The use of trade names in this publication does not imply endorsement by the North Carolina Agricultural Research Service of the products named, nor criticism of similar ones not mentioned. |
Contenido : |
ABSTRACT.
In 1991 and 1992, drip irrigation was used to apply various rates and timings of nitrogen (N) and potassium (K) to black polyethylene plastic‐mulched bell pepper (Capsicum annuum L.) to determine the effect on fruit quality and susceptibility to postharvest bacterial soft rot (Ervinia carotovora subsp. carotovora). Neither rate nor application timing affected total yield in either year. In 1991, the high fertilizer rate (266?309 kg/ha of N and K, respectively) increased class 1 yield in the first harvest and reduced total discards. In 1992, a year with a colder than average spring, the low fertilizer rate (70?81 kg/ha of NK) increased class 1 yield in the first harvest. Mid‐ or late‐season fertigation produced more second harvest yield and less discards than the first harvest in 1991, but not in 1992. Fruits of plants fertilized with high N and K rates had greater N and dry matter concentration. No differences in color or wall thickness at harvest were detected among treatments. Stored fruits turned red slower with the late season low fertilizer rate. There were no differences in rate of weight loss during storage at 10°C. No differences in progression of bacterial soft rot were detected among treatments in 1991, but the higher fertigation rates or late timing application increased resistance in 1992.
Copyright © 1994 by Marcel Dekker, Inc. |
Palabras claves : |
IRRIGATION. |
Thesagro : |
MORRON, PIMIENTO. |
Asunto categoría : |
-- |
Marc : |
LEADER 02565naa a2200205 a 4500 001 1003235 005 2019-01-24 008 1994 bl uuuu u00u1 u #d 024 7 $a10.1080/01904169409364797$2DOI 100 1 $aCARBALLO, S. 245 $aDrip fertigation with nitrogen and potassium and postharvest susceptibility to bacterial soft rot of bell peppers.$h[electronic resource] 260 $c1994 500 $aArticle history: Published online: 21 Nov 2008. Partial funding for this research was from Instituto Nacional de Investigacion Agropecuaria of Uruguay (I.N.I.A.), Uruguay and from the Unites States Department of Agriculture Special Grant F.L. 89-106: "Agricultural Adjustment in the Southeast Through Alternative Cropping Systems." The use of trade names in this publication does not imply endorsement by the North Carolina Agricultural Research Service of the products named, nor criticism of similar ones not mentioned. 520 $aABSTRACT. In 1991 and 1992, drip irrigation was used to apply various rates and timings of nitrogen (N) and potassium (K) to black polyethylene plastic‐mulched bell pepper (Capsicum annuum L.) to determine the effect on fruit quality and susceptibility to postharvest bacterial soft rot (Ervinia carotovora subsp. carotovora). Neither rate nor application timing affected total yield in either year. In 1991, the high fertilizer rate (266?309 kg/ha of N and K, respectively) increased class 1 yield in the first harvest and reduced total discards. In 1992, a year with a colder than average spring, the low fertilizer rate (70?81 kg/ha of NK) increased class 1 yield in the first harvest. Mid‐ or late‐season fertigation produced more second harvest yield and less discards than the first harvest in 1991, but not in 1992. Fruits of plants fertilized with high N and K rates had greater N and dry matter concentration. No differences in color or wall thickness at harvest were detected among treatments. Stored fruits turned red slower with the late season low fertilizer rate. There were no differences in rate of weight loss during storage at 10°C. No differences in progression of bacterial soft rot were detected among treatments in 1991, but the higher fertigation rates or late timing application increased resistance in 1992. Copyright © 1994 by Marcel Dekker, Inc. 650 $aMORRON, PIMIENTO 653 $aIRRIGATION 700 1 $aBLANKENSHIP, S. M. 700 1 $aSANDERS, D. C. 700 1 $aRITCHIE, D. F. 773 $tJournal of Plant Nutrition, 1994, volume 17, Issue 7, pages 1175-1191.
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